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Detection of Ehrlichia canis in domestic cats in the central-western region of Brazil BJM
Braga,Ísis Assis; Santos,Luana Gabriela Ferreira dos; Ramos,Dirceu Guilherme de Souza; Melo,Andréia Lima Tomé; Mestre,Gustavo Leandro da Cruz; Aguiar,Daniel Moura de.
Ehrlichiosis is a worldwide distributed disease caused by different bacteria of the Ehrlichia genus that are transmitted by arthropod vectors. Its occurrence in dogs is considered endemic in several regions of Brazil. Regarding cats, however, few studies have been done and, consequently, there is not enough data available. In order to detect Ehrlichia spp. in cats from the central-western region of Brazil, blood and serum samples were collected from a regional population of 212 individuals originated from the cities of Cuiabá and Várzea Grande. These animals were tested by the Immunofluorescence Assay (IFA) and the Polymerase Chain Reaction (PCR) designed to amplify a 409 bp fragment of the dsb gene. The results obtained show that 88 (41.5%) cats were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ehrlichiosis; Feline; PCR; IFA; Ticks.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200036
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Environmental risk factors for canine toxoplasmosis in a deprived district of Botucatu, SP, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Camossi,LG; Faccioli,PY; Menozzi,BD; Daher,SR; Langoni,H.
Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii that can infect a large variety of animals, including humans. The present study aimed to evaluate the frequency of anti-T.gondii antibodies in dogs from a peripheral district of Botucatu and to establish the association among some epidemiological variables in order to evaluate risk factors for toxoplasmosis infection. Serum samples from dogs were screened using an indirect fluorescent antibody (IFA) test. Anti-T.gondii antibody prevalence was 56%. The highest titer was 1024 (1.79%) and the most frequent titers were 16 (57.14%) and 64 (33.93%). The chi-square (X²) test revealed significant association among variables such as dog access to street, ingestion of raw meat and presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Dogs; IFA; Risk factors.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300006
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Seroepidemiology of Neospora caninum among goats (Capra hircus) in the state of Paraíba, northeastern Brazil Arq. Bras. Med. Vet. Zootec.
Braz,B.M.A.; Valente,J.D.M.; Villalobos,E.M.C.; Lara,M.C.C.S.H.; Machado,C.A.L.; Barbosa,I.C.; Melo,V.S.P.; Stipp,D.T.; Barros-Filho,I.R.; Biondo,A.W.; Vieira,T.S.W.J.; Vieira,R.F.C..
ABSTRACT Although goat dairy farms in Brazil may have a higher risk of infection by Neospora caninum than beef farms, risk factor evaluation on a representative population remains to be fully established in Brazil. Accordingly, this study aimed to establish the occurrence of anti-N. caninum antibodies and factors associated with exposure in 406 blood samples from five dairy and three beef goat farms in the state of Paraíba, northeastern Brazil. Anti-N. caninum antibodies were detected by indirect immunofluorescence assay (IFA), with samples considered positive when reacting with dilution ≥ 1:50. A total of 106/406 goats (26.11%; 95% CI: 21.96-30.72%) were seroreactive comprising 2/61 (3.28%), 10/45 (22.22%), 13/50 (26.00%), 17/51 (33.33%) to 29/46 (63.04%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: IFA; Neosporosis; Seroprevalence; Small ruminants.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100147
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Serosurvey of Rickettsia spp. in small mammals from Mato Grosso do Sul state, Brazil Ciência Rural
Binder,Lina de Campos; Krawczak,Felipe da Silva; Sponchiado,Jonas; Melo,Geruza Leal; Moraes-Filho,Jonas; Bastos,Fernanda Aparecida Nieri; Cáceres,Nilton Carlos; Labruna,Marcelo Bahia.
ABSTRACT: This study aimed to evaluate exposure of wild small mammals to spotted fever group (SFG) rickettsiae in Mato Grosso do Sul State, central-western Brazil. Serum samples of 68 small mammals were analyzed by indirect immunofluorescence assay (IFA) against six Rickettsia species from Brazil. Overall, 37.5% (9/24) marsupials and 6.8% (3/44) small rodents were seroreactive to at least one of the Rickettsia species, with end point titres ranging from 64 to 512. These results suggested that wild small mammals were infected by SFG rickettsiae, and could participate in the ecology of rickettsiae in Mato Grosso do Sul, Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marsupial; Mato Grosso do Sul; Rodent Brazilian spotted fever; IFA.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100453
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